Removing the RAM Disk application will remove all the created RAM disks as well. However, it will not automatically revert Windows temporary folders and Chrome cache to their locations on the physical disk. Just as it didn't move them automatically to the RAM disk when you were installing it.
You should manually revert/restore all the changes you've made when you were moving those files to the RAM disk. And you should do this before uninstalling the RAM Disk application. Otherwise some things may break.